Postdoc Position - Development of Novel Optogenetic Tools

Within the DFG-funded Schwerpunktsprogramm SPP1926 „Next Generation Optogenetics“ ( the following PostDoc position is available in the laboratory of Prof. Peter Hegemann (Hertie Professor for Neuroscience, Experimental Biophysics, Department of Biology, Humboldt-Universität zu Berlin) (
"Development and Application of New Optogenetic Tools Targetted to Intracellular Compartments"
Combination of optogenetic actuators and sensors with targeting motives for synaptic vesicles (SV) or lysosomes. The strategy will be based on recently published work on optogenetic acidification of SV and lysosomes by organelle-specific expression of the light-driven proton pump Arch3. In order to achieve acute depletion of protons from SV and lysosomes we will use a proton channel derived from Arch3, which has been recently developed by the Hegemann lab by point-directed mutagenesis. For the manipulation of luminal chloride we will implement chloride-conductive channelrhodopsins or light-driven chloride pumps. We will characterize and optimize the optogenetic actuators and sensors for subcellular applications beforehand using biophysical approaches. All constructs will be expressed in autaptic neuronal cell cultures using lentivirus or adeno-associated virus as viral gene transfer systems in collaboration with Dr. Benjamin Rost /Charité. Our newly developed tools will allow detailed investigations on the ion homeostasis in the endo-lysosomal compartments in living cells.
The successful candidate should hold a PhD in Biophysics, Biochemistry, or Biology and should have solid experience in molecular biology and electrophysiology. She/he will receive a contract (100% E13 TV-L HU), as well as support with administrative matters, accommodation, visas etc. We invite highly qualified and motivated young scientists of any nationality to apply for the position in our interdisciplinary group.
Please send your application as one single .pdf file with your name not later than August 15th to Dr. Wiebke Apel (


Art des Bewerbungszugangs
Please send application not later than August 15, 2016 as one single .pdf file 
Kontakt für Bewerbungen
Please apply with name and reference number to
Dr. Wiebke Apel ( 

further information at:

Details der Stellenanzeige

Befristete Anstellung
Berufserfahrung vorausgesetzt
Deutschland (Berlin)
10115 Berlin
Biologie & Life Sciences

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