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1 PhD position "Unraveling the transport mechanism of ammonium transport proteins"

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The Research Training Group 2202 „Transport across and into membranes“
at the University of Freiburg (Germany) welcomes applications for

1 PhD position
“Unraveling the transport mechanism of ammonium transport proteins”

Starting date: 01.07.2016
The Research Training Group is funded by the DFG and focuses on the characterization of transport processes at a molecular level. The doctoral researchers examine the transport of ions, metabolites and proteins across and in the cell membrane using a huge variety of biochemical and biophysical techniques.
The three‐year structured program consists of lecture series, seminars, method courses, a summer school, and symposia. In addition, participants will be offered the possibility to attend courses for individual needs. Successful candidates will be awarded the PhD degree (Dr. rer. nat.). The program is taught in English.
The PhD position is open in the in the Institute for Biochemistry, Faculty of Chemistry and Pharmacy in the Andrade research group ( and deals with unraveling the transport mechanism of ammonium transport proteins.
Ammonium Transport (Amt) proteins and their human homologs, the Rhesus proteins, are highly selective transporters for ammonium ions. We have solved the structure of the Archaeoglobus fulgidus Amt1 protein to 1.5 Angstrom and recently established a new electrophysiology technique – solid-supported membrane (SSM)-based electrophysiology – to characterize the electrogenic characteristics of these proteins. As such, we have the necessary tools to dissect the functionality of specific amino acid residues in precise locations of the protein and test various mechanistic hypotheses. In this PhD project, a highly motivated student will be included into a dynamic and friendly team, with state-of-the-art equipment, where he/she will have the chance to learn a variety of biochemical and biophysical tools and produce crucial data to the Amt/Rh proteins field. Moreover, electrophysiology of slow conducting transport proteins is still scarce and therefore also in this regard the data produced will be of interest to a broad range of scientists. The selected student will clone, purify, reconstitute protein in proteoliposomes, carry out electrophysiology experiments, characterize proteoliposomes by freeze-fracture TEM or cryo-EM and solve the three-dimensional structure of interesting variants. Preference will be given to students that have previous experience in protein biochemistry and (eventually) electrophysiology techniques.
  • Diploma, Master or Bachelor’s degree with honours (B.Sc. Hon. for a fast-track application) in Biochemistry, Biology, Biophysics, Chemistry, Physics, Molecular Medicine, Pharmacy or related fields
  • proven qualifications with particular relevance to either transport processes or transport proteins, esp. protein biochemistry or cell biology
  • High motivation and commitment
  • Interest in active participation in the RTG program
  • very good written and spoken English skills
Application documents:
  • Application form (fillable PDF form:
  • Letter of motivation
  • CV
  • Transcripts of studies
  • Two letters of recommendation
  • Proof of fluency in English
The maximum duration of the PhD position will be three years. Further information:


Art des Bewerbungszugangs
Applications should be sent as one pdf file to Please refer to ​"Application RTG 2202​" in the subject line.
Kontakt für Bewerbungen
Coordinator RTG2202:

Details der Stellenanzeige

Befristete Anstellung
Berufserfahrung nicht vorausgesetzt
Deutschland (Baden-Württemberg)
79104 Freiburg
Chemie, Biologie & Life Sciences, Pharma, Physik