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PhD Studentship Position

 Fraunhofer Institute for Cell Therapy and Immunology  Potsdam
PhD Studentship Position


Applications are invited for a PhD studentship from the Department of Cell-free and Cell-based Bioproduction, Fraunhofer Institute for Cell Therapy and Immunology IZI, Potsdam, Germany to work on cell-free synthesis and functional analysis of membrane proteins.

Project description:
Ion-channels play a basic role in pain causing stimuli (TRP channels), epithelial chloride transport in many organs (CFTR), c-type inactivation (voltage gated potassium channels), neurotransmitter release and regulating contractile tone (BK channels), mitochondrial-membrane transport (VDAC). Any functional defect in the MPs could affect the cellular activities which could often lead to a wide range of diseases like cystic fibrosis, cancer, pain, autoimmune diseases, stroke, apoptosis etc. Cell-free protein synthesis (CFPS) is a versatile, flexible, faster and alternative approach for the synthesis of difficult-to-express proteins including membrane proteins and toxic proteins. CFPS has a high degree of controllability and provides a completely open system allowing direct manipulation of the reaction conditions to optimize protein folding, disulfide bond formation, and incorporation of noncanonical amino acids. One can add the nanodiscs, detergents or make use of the native endogenous microsomes for the solubilization and functional folding of the MPs. Thus efficient use of CFPS helps in the economic production of functional MPs as targets for drug development.
The goal of this project is to synthesize Ion-channels using cell-free systems based on eukaryotic and prokaryotic extracts and develop functional and pharmacological assays. The main focus will be on the investigation of gating and function of integral membrane proteins; in particular ion-channels (Potassium channels, Anionic channels, Transient receptor potential channels, Toxins and Exchangers).

Location:
This work will be carried out at the Department of Cell-free and Cell-based Bioproduction, Fraunhofer Institute for Cell Therapy and Immunology IZI-BB situated in the scientific park at Potsdam-Golm. The department is specialized in prokaryotic and eukaryotic cell-free systems. The department is equipped with all the necessary facilities (Molecular biology, Protein synthesis, Electrophysiology, Cell-culture, Fluorescence microscopy labs) for conducting research.

Qualifications:
Applicant should have good academic records with a masters or diploma in Pharmacology, Pharmacy, Biophysics, or Biochemistry. Candidates with experience or interest in Liposomes, Electrophysiology, and Functional characterization of membrane proteins will be favored. Applicant should have excellent English language skills.

Position:
Payment will be based on salary grade TVöD (50%). Funding is available for three years.
 
How to apply:
Application: Please Send CV, cover letter, transcripts with a brief summary of previous research work and 2 references to Dr.Stefan Kubick, Head of Department, Cell-free and Cell-based Bioproduction, Fraunhofer Institute for Cell Therapy and Immunology (IZI), Branch Bioanalytics and Bioprocesses Potsdam-Golm (IZI-BB), Science Campus Golm, Am Mühlenberg 13, 14476 Potsdam, Germany +49-33158187306, stefan.kubick@izi-bb.fraunhofer.de



 
Kontakt für Bewerbungen
Stefan Kubick
+49-33158187306, stefan.kubick@izi-bb.fraunhofer.de
Science Campus Golm, Am Mühlenberg 13, 14476 Potsdam, Germany
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