PhD Studentship Position

PhD Studentship Position


Applications are invited for a PhD studentship from the Department of Cell-free and Cell-based Bioproduction, Fraunhofer Institute for Cell Therapy and Immunology IZI, Potsdam, Germany to work on cell-free synthesis and functional analysis of membrane transporter proteins.

Project description:
Membrane transporter proteins play a basic role in the absorption of dietary di- and tripeptides from the small intestinal lumen in a proton dependent way (PEPT1 transporter), hepatic uptake of several important drugs and small molecules (OCT1), drug absorption and disposition many drugs (PGP1 or MDR1), glucose transport across membranes (SGLT1), regulating excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system (EAAT). Any functional defect or overexpression of these proteins could affect the cellular activities which could often lead to a wide range of disorders like intestinal inflammation, glucose-galactose malabsorption, hepatic steatosis, amyotrophic lateral sclerosis (ALS), unexpected toxicities or drug-drug interactions. Cell-free protein synthesis (CFPS) is a versatile, flexible, faster and alternative approach for the synthesis of difficult-to-express proteins including membrane proteins and toxic proteins. CFPS has a high degree of controllability and provides a completely open system allowing direct manipulation of the reaction conditions to optimize protein folding, disulfide bond formation, and incorporation of noncanonical amino acids. One can add the detergents, liposomes or make use of the native endogenous microsomes for the solubilization and functional folding of the MPs. Thus efficient use of CFPS helps in the economic production of functional MPs as targets for drug development.

The goal of this project is to synthesize transporters using cell-free systems based on eukaryotic and prokaryotic extracts and develop functional and pharmacological assays. The main focus will be on the investigation of clinically significant transporters (Peptide transporter, Glucose transporter, Aminoacid transporter, Cationic transporter, Dopamine and serotonin transporters, and Multidrug transporter).

Location:
This work will be carried out at the Department of Cell-free and Cell-based Bioproduction, Fraunhofer Institute for Cell Therapy and Immunology IZI-BB situated in the scientific park at Potsdam-Golm. The department is specialized in prokaryotic and eukaryotic cell-free systems. The department is equipped with all the necessary facilities (Molecular biology, Protein synthesis, Electrophysiology set up for transporter analysis, cell-culture, and fluorescence microscopy labs) for conducting research.

Qualifications:
Applicant should have good academic records with a masters or diploma in Pharmacology, Pharmacy, Biophysics, or Biochemistry. Candidates with experience or interest in Liposomes, Transporter assays, Electrophysiology, and Functional characterization of membrane proteins will be favored. Applicant should have excellent english language skills.

Position:
Payment will be based on salary grade TVöD (50%). Funding is available for three years.
 
How to apply:
Application: Please Send CV, cover letter, transcripts with a brief summary of previous research work and 2 references to Dr.Stefan Kubick, Head of Department, Cell-free and Cell-based Bioproduction, Fraunhofer Institute for Cell Therapy and Immunology (IZI), Branch Bioanalytics and Bioprocesses Potsdam-Golm (IZI-BB), Science Campus Golm, Am Mühlenberg 13, 14476 Potsdam, Germany +49-33158187306, stefan.kubick@izi-bb.fraunhofer.de
 

Kontaktdaten


Kontakt für Bewerbungen
Stefan Kubick
+49-33158187306, stefan.kubick@izi-bb.fraunhofer.de
Science Campus Golm, Am Mühlenberg 13, 14476 Potsdam, Germany

Details der Stellenanzeige


Arbeitszeit
Vollzeit
Vertragslaufzeit
Befristete Anstellung
Stellentyp
Promotionsstelle
Berufserfahrung
Berufserfahrung vorausgesetzt
Region
Deutschland (Brandenburg)
Arbeitsort
14476 Potsdam
Fachgebiet
Pharma, Healthcare & Gesundheitswesen, Humanmedizin, Chemie, Physik, Medizintechnik, Pharmatechnik
JETZT BEWERBEN

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