Universität des Saarlandes

Institut für Molekulare Zellbiologie

We are mainly interested in cellular and/or subcellular mechanisms of signal transduction mechanisms in the heart using calcium ions, DAG and InsP3 as second messengers that are involved in the deseased heart. In particular, we investigate spatio-temporal aspects of G-protein-activated signalling via PKCs and PLCs by realtime and ultrafast confocal microscopy utilizing FRET and FLIM methods to study e.g. phosphorylation events or protein/protein interactions. Furthermore we are actively investigating gene-expression patterns of PKCs, InsP3Rs and PLCs the changes thereof during various cardiac pathologies using single-cell models and patient samples by e.g. applying quantitative realtime PCR, RT-PCR and Western-blotting techniques. In addition to those analytical techniques we have developed an adenoviral gene transfer for our primary end-differentiated adult cardiac myocytes.

Details


Adresse:
Universität des Saarlandes
Institut für Molekulare Zellbiologie
Gebäude 61
66421 Homburg
Deutschland
Arbeitsgebiet:
Forschung & Entwicklung
Forschung & Lehre
Expansion:
international
Mitarbeiteranzahl:
11-50
Gründungsjahr:
2002