PhD Position in Role of the hypoxia-inducible factor-1α in metabolic changes in the hypothalamus of obese mice

The researchers in the DFG-funded Graduiertenkolleg 1957 study the effects of adipose tissue hormones on CNS function and in turn, the control of adipose tissue physiology by the brain. We are looking for highly motivated candidates for the PhD project (see title).
Tentative start: May 2020. Earlier start date could be discussed with the PIs in the interview.

Activities and responsibilities

Project Description:

Astrocyte glucose metabolism is essential for the delivery of energy substrates to neurons and may regulate a variety of brain functions. The transcription factor hypoxia-inducible factor-1α (HIF-1α) regulates astrocytic glycolysis and lactate production (J Neurosci 32,9727, 2012). In obesity, low-grade inflammation occurs within the hypothalamus and astrocyte activity as well as glucose uptake is changed. Using in vitro and in vivo systems we will analyze the effect of dietary and inflammatory factors on HIF-1 signaling and target gene regulation in cultured astrocytes and within the hypothalamus of obese mice.

Experimental Methods:
  • Primary cell cultures (astrocytes, microglia, brain endothelial cells), co-culture systems
  • Mouse experiments including of rAAV vectors application and phenotyping (energy expenditure, body weight)
  • DNA and RNA extraction, PCR, qPCR
  • Western blotting, immunocytochemistry
  • Chromatin Immunoprecipitation (ChIP), pyrosequencing of DNA methylation, reporter gene assays

Qualification profile

  • Research-based master's degree or equivalent (e.g. diploma) passed with an above-average grade in biology, biochemistry, molecular life science, neuroscience, nutrition & biomedicine, pharmacology or a related subject
  • Excellent English language skills in speaking and writing
  • Pro-active attitude, good communication skills and ability to work independently in an interdisciplinary team

Send application to

GRK1957 Office, chaoqun.jiang(at)uni-luebeck.de

https://www.grk1957.uni-luebeck.de/grk-1957/phd-positions-on-adipocyte-brain-crosstalk.html

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Über University of Lübeck

Welcome at the GRK1957 "Adipocyte-Brain Crosstalk"Our DFG funded  Graduiertenkolleg 1957  offers focused research projects and a structured training programme. In an interdisciplinary research approach we address the effects of hormones derived from adipose tissue (adipokines) on CNS function and, in turn, the control of adipose tissue and body weight by the brain. ...
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